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Development and validation of DNA-based diagnostic techniques with particular reference to bivalve mollusc pathogens ArchiMer
Berthe, Franck.
The International Aquatic Animal Health Code of the OIE (the World Organization for Animal Health) includes serious pathogens that have been causing important losses in the mollusc aquaculture industry throughout the world. This list also meets the ones established by the European Union regulation.
Tipo: Text Palavras-chave: Pathogens; Techniques; Diagnostics; Pathology; Molluscs.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3213.pdf
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Development of mussel-specific expression vectors suitable for transgenic organisms: implication on the establishment of mollusc continuous cell lines ArchiMer
Delsert, Claude; Cancela Da Fonseca, Leonor.
The main objective of this project was to develop the tools for the in-depth understanding of mollusc biology and pathology. Such a goal has been reached in other biological systems through the development of molecular biology and cell culture. Indeed, development of a mollusc cell culture system is an essential step to approach many of the problems related to mollusc pathology, in particular to use molecular biology tools to study viral infection of these organisms. No continous cell line is available from bivalves and while cell cultures have been obtained from these organisms they are difficult to develop and labor intensive, survival cultures being maintained only for a few weeks. Our goal has been to study in detail many of the parameters involved in...
Tipo: Text Palavras-chave: Pathology; Mussel-specific expression vectors; Cell culture; Mytilus galloprovincialis; Gene; Genetic marker.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00044/15540/12917.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - Final Report 4th January 1999 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular immunological and cellular tools "VINO". Second periodic progress report: 4th January 2000 at 3rd January 2001 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using teclmiques developed by the patiners to characterise viruses by studying their genome and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpesvirus (OsHV-1) DNA sequence with determination of the genome structure. 2 - Comparing OsHV -1 with viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure. 3 -...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00048/15947/13377.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf
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Does the prevalence of Bonamia and Marteilia diseases be reduced on flat oysters (Ostrea edulis) of Atlantic and Mediterranean origin, when they are reared together with the Japanese oyster (Crassostrea gigas) in tidal ponds? ArchiMer
Bodoy, Alain; Bougrier, Serge; Geairon, Philippe; Garnier, Jacqueline; Boulo, Viviane; Heurtebise, Serge.
The flat oyster Ostrea edulis was originally reared in several regions of the European waters. The tidal saline ponds on the Atlantic coasts of France were highly productive before being hit by two consecutive epizooties. Parasitic diseases were described on this species, both of them leading 10 economic losses, as heavy mortalities have usually been recorded before the oysters reach the commercial size. Repeated experiments showed that the two diseases, Marteilia refringens and Bonamia ostreae are still active in this environment. Recently, some evidences were given that haemocytoblasts of Crassostrea gigas had the ability to lyze the Bonamia cells. Therefore, in an experiment of contiguous culture of the two species of oysters, the growth, mortality and...
Tipo: Text Palavras-chave: Molluscs; Mortalities; Growth; Pathology; Crassostra gigas; Ostrea edulis; Marteilia refringens; Bonamia ostreae.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3049.pdf
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Effect du Vibrio P1, pathogene de Ruditapes philippinarum , sur d'autres especes de bivalves ArchiMer
Maes, P; Paillard, C.
The symptom which characterizes the brown ring disease (BRD) in Manilla clam Ruditapes philippinarum has been observed in other Bivalvia species (Veneridae and Pectinidae). Vibrio P1, responsible of the BRD in R. philippinarum , has been detected in other Veneridae which were carrying the symptom. In mussel and oysters which are cultivated in Europe, the symptom has never been reported and Vibrio P1 has not been detected. Experimental contaminations of bivalves of commercial interest with the pathogen showed a clear Vibrio P1 sensitivity of European clam R. decussatus ; this latter seems however to be less sensitive than the Manilla clam.
Tipo: Text Palavras-chave: Ruditapes philippinarum; Bivalvia; Diseases; Marine molluscs; Pathology; Vibriosis.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-926.pdf
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Effects of Corticosteroids in Stray Cats with Natural Antibodies to Toxoplasma gondii OAK
Samad, Md Abdus; Islam, Md Rafiqul; Dey, Badal Chandra; Alam, Md Mahabub.
Palavras-chave: Pathology; Seropositive cats; Immunosuppression; Oocyst shedding.
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/255
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Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882) Baylis, 1920 in rabbits Arq. Bras. Med. Vet. Zootec.
Barros,L.A.; Tortelly,R.; Pinto,R.M.; Gomes,D.C..
Rabbits were infected per os with 10 Eustrongylides ignotus L4 and with 50 Contracaecum multipapillatum L3 per rabbit, recovered from naturally infected freshwater fishes (Hoplias malabaricus) in order to evaluate the patogenicity of these two nematode species in mammalian host. Two rabbits (20%) infected with E. ignotus died before the fourth day post-inoculation (one after 51 and the other after 78 hours). Six rabbits (60%) were inappetent until the fifth day following experimental inoculation. No clinical signs in rabbits inoculated with C. multipapillatum were observed; nevertheless, eight (80%) animals were positive for this nematode species. Rabbits inoculated with E. ignotus, had gastric congestion with hematoma of the gastric wall in 60% of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eustrongilydes ignotus; Contracaecum multipapillatum; Experimental infection; Rabbits; Pathology.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300007
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Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf
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Eficácia de três vermífugos contra a verminose gastrointestinal dos caprinos. Infoteca-e
CHARLES, T. N. P.; POMPEU, J.; MIRANDA, D. B. de.
Testes feitos no Campo Experimental de Mandacaru da Caatinga do CPATSA, para verificar a eficácia dos vermífugos usados contra verminose gastrointestinal dos caprinos. O teste foi direcionado a verminose causada por vermes redondos (nematodeos) uma vez que as infecções causadas por vermes chatos (cestiodeos) e verme em forma de folha (trematodeos) são de pouca importância nas regiões secas.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Patologia animal; Vermífugo; Gastrointestinal; Verme; Control; Pathology; Caprine.; Caprino; Parasitose; Verminose.; Sheep..
Ano: 1989 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131764
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Essai de culture de l'huître plate Ostrea edulis dans le bassin d'Arcachon. Résultats préliminaires ArchiMer
Robert, Rene; Pichot, Yves; Comps, Michel.
From 1988 to 1989, a study has been conducted pertaining to the growth of the flat oyster Ostrea edulis, as well as Marteilia refringens and Bonamia ostreae outbreaks. Commercial mono specific farming (flat oysters), with low density or mixed (flat and hollow oysters) along with higher density, has been taking place in the Arcachon basin oceanographic sectors, where main hydrobiological parameters were acquired simultaneously. The excellent growth of the flat oyster was witnessed in mono specific farming. However this growth was average in mixed farming, probably due to higher total oyster density and interspecies competition. Regardless of the type of farming, Marteilia refringens infection was very quick and may have been related to climatic bay water...
Tipo: Text Palavras-chave: Basin of Arcachon; Pathology; Bonamia ostreae; Marteilia refringens; Growth; Ostrea edulis; Bassin d'Arcachon; Pathologie; Bonamia ostreae; Marteilia refringens; Croissance; Ostrea edulis.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/rapport-1592.pdf
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Estudio epizootiológico de Penaeus vannamei en tres fincas camaroneras del Caribe colombiano OceanDocs
Vallejo-Isaza, A.; Newmark-U., F..
An epizootiological study of the mayor deseases affecting the penaeid shrimp Penaeus vannamei was performed from june 1994 throuh august, 1995 in tree farms located on the colombian Carebbean coast. The presence and prevalence of infectious agents such as bacteria, protozoa, viral inclusions bodies and changes histopatologicals was determinated by means of gross examination and histological analysis.. The relationship between these infection agents and specific disease was also studies. The presence of some viruses such a Baculovirus penaei, IHHNV, TSV and LOVV, were determinated by demostration of the cytopathical effects characteristic of these agent. Additionaly, a logistic regression analysis was used to determine the relationship between some clinical...
Tipo: Journal Contribution Palavras-chave: Shrimp culture; Pathology; Pathology; Http://aims.fao.org/aos/agrovoc/c_5631.
Ano: 1999 URL: http://hdl.handle.net/1834/3383
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Etude des effets de polluants sur les paramètres hémocytaires de l'huître creuse, Crassostrea gigas - Interactions entre environnement, mécanismes de défense et maladies infectieuses ArchiMer
Gagnaire, Beatrice.
Shellfish industry is mostly realized in estuary zones, which are subjected to pollutions due to anthropic activities. The harmful effects of pollutants on animals inhabiting these estuarine zones are poorly known. Among these animals, Pacific oyster, Crassostrea gigas, may represent a model because they are sedentary and they filter water intensively. Among all physiological functions possibly disturbed by pollutants, defence mechanisms are poorly studied in bivalves. Moreover, animals presenting impaired defence mechanisms may be more sensitive to infectious diseases. In this context, effects of pollutants on hemocyte functions and on sensitiveness to diseases were tested in C. gigas. After adjusting the protocols for the monitoring of hemocyte...
Tipo: Text Palavras-chave: Real time PCR; Flow cytometry; Pesticides; Pollutants; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster; Pathology; Immunotoxicology; PCR en temps réel; Cytométrie de flux; Pesticides; Polluants; Phagocytose; Hémocytes; Crassostrea gigas; Huître creuse; Pathologie; Immunotoxicologie.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-730.pdf
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Étude des interactions entre infection à ostreid herpesvirus 1, immunité, autophagie et pesticides chez l’Huître creuse, Crassostrea gigas ArchiMer
Moreau, Pierrick.
The thesis work is part of the very current issue on mass mortality outbreaks affecting Pacific oyster, C. gigas, spat and juveniles and questions about the involvement of pesticides in this phenomenon. The first part of this thesis was devoted to study the effects of pesticides on hemocyte parameters in the Pacific oyster (in vitro and in vivo). The immunomodulator effect of selected pesticides (alone or in mixture) has been explored principally through flow cytometry. The second part concerned the study of the effects of a pesticides mixture on OsHV-1 itself. No direct effects have been reported on the viral particles in presence of the pollutants. Moreover, pesticide effects on Pacific oysters were also explored through experimental pathology assays...
Tipo: Text Palavras-chave: Pacific oyster; Pesticides; OsHV-1; Autophagy; Immunity; Hemocyte; Pathology.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00226/33740/32149.pdf
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Etudes pathologiques et epidemiologiques des lesions parasitaires chez Ostrea edulis : Premiers resultats d'un recherche prospective comparative chez les principales especes de mollusques des zones ostreicoles de Bretagne nord ArchiMer
Poder, M; Auffret, Mathieu; Balouet, G.
This paper is a contribution to the study of the Bonamia ostreae epizooty in french european oyster Ostrea edulis : parasite process (hemocytic parasitosis); experimental contaminations on parks and in tanks. First results of an epidemiological watch conducted in oyster culture sites show in molluks mytilidae, pectinidae, cardiidae, veneridae: the absence of Bonamia ostreae forms; the existence of different types of parasitism or commensalisus of inequal pathological importance and especially a parasite of the Marteilia genus in Tapes sp . digestive epithelium. Additionally, a new example of hemocytosercoma is observed in Cerastoderma edule .
Tipo: Text Palavras-chave: Ruditapes philippinarum; Tapes rhomboides; Tapes pullastra; Mytilus edulis; Cerastoderma edule; Ostrea edulis; Bivalvia; Marteilia; Bonamia ostreae; Comparative studies; Parasitic diseases; Commensalism; Parasitology; Marine mollusks; Epidemiology; Pathology; Pathologie; Mollusques; Bonamia ostreae; Epizootie; Ostrea edulis.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1182.pdf
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Etudes sur la pathologie des huîtres ArchiMer
Balouet, G..
French summary: In 1974, at the request of a group of oyster farmers in the Brittany region, the Pathology Laboratory of the U.E.R. Medicine of Brest undertook a series of theoretical and applied research projects concerning the problem of flat oyster digestive gland disease, due to Marteilia refringens, the primary economic problem of that time. In 1978, in the research report of Contract C.N.E.X.O. 77/1690, the main results obtained since the beginning of the study of the parasitosis were made public. The present report has as its objective to show: - The results obtained in 1978-1979 while monitoring the parasitosis, and most especially the epidemiological evolution of the disease; - Information concerning the presence of other infectious diseases...
Tipo: Text Palavras-chave: Parasite; Ostrea edulis; Oyster; Pathology.
Ano: 1978 URL: http://archimer.ifremer.fr/doc/1978/rapport-1927.pdf
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Evolution et estimation des mortalités estivales de naissain d'huître creuse (Crassostrea gigas) en baie de Quiberon, de mai à septembre 1998. ArchiMer
Martin, Anne-genevieve; Fleury, Pierre-gildas; Tige, Gilbert; Hirata, Toshio; Le Coguic, Marie-jo; Langlade, Aime; Mazurie, Joseph; Ostréiculteurs de la baie de Quiberon.
La question des mortalités estivales de naissains d'huîtres creuses en baie de Quiberon a été abordée selon deux approches complémentaires, l'une plus temporelle, avec le suivi estival régulier de quelques semis, l'autre plus spatiale avec une estimation en plongée des survies en fin d'été d'un plus grand nombre de semis répartis à travers la baie. Le suivi de l'évolution des semis a permis de dater et de quantifier les mortalités estivales sur plusieurs périodes (juin et août essentiellement) ; mais il n'a pas permis d'en identifier les causes. Le rôle des pathogènes, notamment, n'est pas défini. Par ailleurs, l'estimation spatiale des mortalités en fin d'été met en évidence des mortalités nettement différenciées entre les semis (entre 6 et 48%). Parmi...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Naissain; Quiberon; Mortalité; Croissance; Vitalité; Pathologie; Herpès-virus; Pacifie cupped oyster; Crassostrea gigas; Spat; Quiberon (France); Mortality; Growth; Vitality; Pathology; Herpes-virus.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00102/21326/18949.pdf
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Facteurs de mortalité observés chez les tortues marines dans le golfe de Gascogne ArchiMer
Duguy, R; Moriniere, P; Le Milinaire, C.
Factors of mortality in marine turtles near the French coast of the Bay of Biscay have been recorded for four species: leatherback (Dermochelys coriacea), loggerhead (Caretta caretta), Ridley's turtle (Lepidochelys kempii) and green turtle (Chelonia mydas). Most accidental captures of D. coriacea by fishing gears lead to drowning, but the main threat for the species is the ingestion of floating waste. Plastic bags induce occlusion or lesions in the stomach and subsequent death. For C. caretta, L. kempii and C. mydas, the pathology points out mainly two features: external or internal mycosis.
Tipo: Text Palavras-chave: Chélonien; Tortue marine; Pathologie; Pollution; Golfe de Gascogne; Chelonian; Marine turtle; Pathology; Pollution; Bay of Biscay.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00326/43693/43236.pdf
Registros recuperados: 142
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